Immunohistochemical Evaluation of Hemostatic Changes in Glioblastoma Multiforme and Low-Grade Astrocytoma

Özet

AIM: To compare glioblastoma multiforme with astrocytoma grade II by subjectively evaluating the levels of prothrombin andbiotinylation thrombin, and G protein serum protease activatin receptors, as tissue factors causing hypercoagulation and affectingcoagulation.MATERIAL and METHODS: Specimens from 35 cases with glioblastoma multiforme and 23 cases with astrocytoma grade IIwere evaluated immunohistochemically. The specimens were stained with hematoxylen-eosin and immunohistochemically forprothrombin, biotinylation thrombin and protease activating factor receptors to determine the correlation between the tumormalignancy and coagulation factor receptors.RESULTS: An increase in malignancy was seen to result in an increase in prothrombin, biotinylation thrombin, protein activatorreceptor 1, protein activator receptor 3, and protein activator receptor 4 levels, and a decrease in protein activator receptor 2level. These data showed that there was hypercoagulability in glioblastoma multiforme. Descriptive statistics and Mann-Whitney Uanalysis were used to evaluate the results.CONCLUSION: In glioblastoma multiforme with no radiological evidence of hemorrhage, low molecular weight heparin should beadministered peroperatively and continued for 3 months postoperatively to prevent the development of deep venous thrombosis.This will also be useful in the prevention of invasion, angiogenesis, metastasis and tumour progression.