INVESTIGATION OF PROTECTIVE EFFECTS OF DIFFERENT DOSES OF OMEGA 3 FATTY ACIDS IN EXPERIMENTAL RENAL ISCHEMIA REPERFUSION DAMAGE

Özet

ObjectiveIschemia reperfusion (IR) injury is caused by a suddenand temporary impairment of blood flow to a particularorgan or tissue. Renal IR-induced kidney damage causeshigh levels of permanent damage and mortality.Dietary omega-3 fatty acids have anti-inflammatoryand immune-modulating effects and are used as protectiveagents.Materials and MethodsIn the study; Protective effects of different doses ofomega-3 fatty acid in ischemia reperfusion injury wereinvestigated by biochemical and histological analyzes.40 adult female rats were divided into 5 groupsas sham control, IR, IR+100, IR+400, IR+700 (n:8).The right kidney was removed by nephrectomy in thesham control group, and the left kidney was not treated.For IR injury; the right kidney was removed bynephrectomy, and the left kidney was clamped. Omega-3 fatty acids of 100 mg/kg, 400 mg/kg and 700 mg/kg were administered by gastric gavage to the treatmentgroups for 14 days before the surgery and for15. gastric gavage was administrated in the morningof the operation.ResultsAccording these data; with application of omega-3,histopathological examination results were correlated.It is found that, histopathological results weresupported by biochemical results. There was a significantdecrease in malondialdehyde (MDA) and myeloperoxidase(MPO) levels in the treated groups, whilecatalase (CAT) and glutathione peroxidase (GSH-Px)levels were detected to be increased.ConclusionIt was concluded that administration of omega-3 fattyacids beforehand in cases that may cause ischemiaand reperfusion damage in the kidney can provide aprotective effect on this tissue.