Effects of Zinc Oxide on mRNA Expression of Genes in Brain Tissue of Rats

Özet

AIM: To investigate the expression of the DR4, DR5, OPG, DcR1, and DcR2 in rat brain tissue.MATERIAL and METHODS: Thirty rats were used in this study. The rats were divided into three groups as the control group (n=10),tumor group (n=10), and zincoxide (ZnO) nanoparticles (NP) treatment group (n=10). The reverse transcription polymerase chainreaction (RT-PCR) and Western Blotting methods were used to measure the expression of DR4, DR5, OPG, DcR1, DcR2 and β-actinin the brain tissues of all the three groups.RESULTS: Expression of DR4, DR5, OPG, DcR1, and DcR2 genes were decreased in the tumor group. Overexpression of DR4,DR5, OPG, DcR1, and DcR2 was observed in brain tissues of the ZnO-NP group.CONCLUSION: Increased expression of the DR4, DR5, OPG, DcR1, and DcR2 genes may play an important role in ZnO-NPtreatment of brain tumors.