Immunohistochemical investigation of lipid peroxidation in renal coccidiosis of geese

Özet

Objective: In this study, we aimed to evaluate the oxidative damage caused by lipid peroxidation due torenal coccidiosis by histopathological and immunohistochemical methods.Material-Method: The material of this study was made up of tissue samples taken from 139 geese whoseaverage age was 10 weeks, who were brought to our department dead between 2013-2020. Tissue samplestaken were fixed in 10% buffered formaldehyde solution. 5 µm-thick sections were taken from the paraffinblocks prepared after routine tissue follow-up procedures. Hematoxylin & Eosin staining was applied tothe sections in order to detect histopathological changes. Sections were examined and photographed undera light microscope.Results: Various clinical signs such as fever, respiratory distress, weakness, anorexia, tremors, inability toget up from the ground, balance disorders, rotational movement, diarrhea, wheezing were detected ingeese. In systemic necropsies of geese, large and small white nodular structures were detected in the kidney.In histopathological examinations, coccidiosis agents (E. truncata) were found in the tubular epithelium ofthe kidney. Necrosis and mononuclear cell infiltration were observed in the tubules due to the presence ofE. truncata. In addition, edema and hyperemia in the lungs, multifocal necrosis in the liver, cell infiltrationin the portal spaces and enteritis were other important histopathological findings. In one case, aspergillosiswas detected together with renal coccidiosis. We observed that MDA expression was more severe in oocyststages, which is the mature form of the parasite, compared to other parasitic life stages.Conclusion: Based on the results obtained from this study, it was revealed that renal coccidiosis in geesecaused lipid peroxidation / oxidative damage through the increase in MDA expression.