Protective Role of Dexmedetomidine on Ileum and Kidney Damage Caused by Mesenchymal Ischaemia in Rats

Özet

Objective: The aim of this study was to demonstrate ischaemia reperfusion (IR) injury on the ileum and kidney tissue in rats and toevaluate the effect of dexmedetomidine administered at different doses and dosing schedules on recovery.Methods: A total of 30 rats were randomly divided into five groups. Group I: sham; Group II: control; Group III: dexmedetomidinebefore ischaemia; Group IV: dexmedetomidine after ischaemia; and Group V: dexmedetomidine before and after ischaemia. The malondialdehyde(MDA) and signal peptide-CUB-EGF (epidermal growth factor) domain-containing protein 1 (SCUBE-1) levels of all subjectswere studied from the serum, ileum, and kidney tissues. Moreover, the histopathology of ileum and kidney tissues was examined.Results: The SCUBE-1 levels were found to be highly similar to the MDA levels in ischaemic groups. The serum SCUBE-1 levels obtainedwere significantly lower in Group V compared to Groups II, III and IV (p<0.001, p=0.003, p=0.013, respectively). The apoptosisindexes were found to be lower in groups receiving dexmedetomidine compared to Group II. The groups receiving dexmedetomidinewere detected to have normal morphological appearance when compared to Group II.Conclusion: In this study, the use of dexmedetomidine in the preoperative and peroperative periods may be beneficial in reducing thenegative effects of IR injury.