Journals / Turkish Journal of Cancer / 2001 / Cilt: 31 - Sayı: 1
Comparative genomic hybridization of primary skeletal Ewing's sarcoma
- Journal
- Turkish Journal of Cancer
- Pages
- 21–26
- DOI
- —
Abstract
We used comparative genomic hybridization (CGH) technique to identify the genetic changes in 8 primary skeletal Ewing's sarcoma samples from archieved paraffin-embedded tissues from children (4-17 years old) using double step degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). CGH analysis showed t h at the most common genetic alterations were losses of Chromosomes 1p, 2q, 3p, 14q, 21, X and gains of Chromosomes 8q and 17q. Chromosomal deletions were found only in samples taken from males, while amplifications were identified in both sexes. As a result, this difference may explain why tumour occurs about 1.5 times more commonly in males than in females.
Özet
Comparative genomic hybridization of primary skeletal Ewing's sarcoma