Effects of immobilization and whole-body vibration on rat serum TypeI collagen turnover

Abstract

Objective:The aim of this study was to investigate the effects of short-term, high-magnitude whole-bodyvibration (WBV) on serum type I collagen turnover in immobilized rats.Materials and Methods:Thirty Wistar albino rats were randomly divided into the following 5 groups:immobilization (IS), immobilization?remobilization (IR), immobilization?WBV (IV), control (C), andWBV control (CV). Immobilization was achieved by casting from the crista iliaca anterior superior to thelower part of the foot for 2 weeks. The applied WBV protocol involved a frequency of 45 Hz andamplitude of 3 mm for 7 days starting a day after the end of the immobilization period. Serum type Icollagen turnover markers were measured by using ELISA kits.Results:Serum NH2-terminal propeptide of type I collagen (PINP) levels were significantly lower in theimmobilization groups (p<0.02) compared with the control groups. Although WBV improved PINPlevels in the control groups, there were no differences in PINP levels among the immobilization groups.Similarly, serum COOH-terminal telopeptide of type I collagen (CTX) levels were higher in the WBVcontrols than their own controls (p<0,05). Immobilization led to deterioration of tendon tissue, asobserved by histopathological analysis with a transmission electron microscope.Conclusion:Although 1 week of WBV had a positive effect on type I collagen turnover in controls, it is notan efficient method for repairing tissue damage in the early stage following immobilization.©2016 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V. This isan open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)