The role of ADAMTS genes in the end stage of hip osteoarthritis

Özet

Purpose: The aim of this study is to investigate which ADAMTS genes play a major role in the development of primary hip osteoarthritis, by comparing the tissue and blood samples in patients with hiposteoarthritis and a control group.Material and methods: Human articular cartilage was obtained from femoral heads of 15 patients withend stage osteoarthritis undergoing total hip replacement. As the control group, the cartilages wasobtained from femoral heads of 15 patients, who did not have osteoarthritis or degenerative changes inhip joint, undergoing hip replacement following the fracture of the femoral neck. After the cartilagesamples were taken from the resection materials, the DNA polymorphisms in the patients' cartilagesamples were tested by Polymerase Chain Reaction (PCR), the serum levels of aggrecanase genes wereanalyzed with Enzyme-Linked ImmunoSorbent Assay (ELISA).Results: The level of ADAMTS5 and ADAMTS9 genes were found significantly lower as a result of ELISAanalysis degenerative arthritis group than the control group (p 0,05). As a result of quantitative real time RT-PCR analysis,the level of ADAMTS8 mRNA increased 3.5 fold in hip degenerative arthritis group when compared withfemoral neck fractures group. ADAMTS1, ADAMTS4 and ADAMTS5 expression levels in hip degenerativearthritis group were decreased 2.5, 2 and 2.5 fold, respectively. ADAMTS9, 15 were found to be similarbetween two groups.Concluson: As a result of this study on hip osteoarthritis, the ADAMTS8 levels was found to be significantly higher in the end stage of hip osteoarthritis. Unlike similar studies on knee osteoarthritis,ADAMTS1,4,5 levels were found to be lower.