Antimicrobial, Larvicidal Activities and Composition of the Leaf Essential Oil of Magnolia coco (Lour.) DC

Özet

The chemical constituents, antimicrobial and larvicidal activities of essential oils obtained byhydrodistillation of the leaves of Magnolia coco (Lour.) DC. are being reported. The essential oils were analyzedusing Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GCMS)while the larvicidal activity was evaluated by the protocol of the World Health Organization (WHO). Themicrobroth dilution assay was used for the study of antimicrobial activity. The major compounds in the oil weresabinene (35.4%) and β-pinene (16.3%). The oil exhibited 100% mortality towards Aedes albopictus at 24 h andconcentrate of 50 g/mL while maximum mortality was shown against Ae. aegypti and Culex quinquefasciatus atconcentration of 100 g/mL. The median lethal concentrations, LC50, values of 11.01, 46.46 and 87.61 g/mLwere obtained against Ae. albopictus, Ae. aegypti and Cx. quinquefasciatus respectively at 24 h. However, the LC50values at 48 h period were 10.40, 41.98 and 53.86 g/mL respectively. In addition, the oil exhibited antimicrobialactivity against Enterococcus faecalis ATCC 299212, Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC14579 and Candida albicans ATCC 10231 with minimum inhibitory concentrations (MIC) values of 128.0, 256.0,128.0 and 64.0 μg/mL respectively, while the IC50 values were 64.33, 128.4, 65.33 and 32.33 μg/mL respectively.These reports, the first of its kind, indicate the potentials of M. coco leaf oil as a source of antimicrobial andlarvicidal agents.