DETECTION OF SOME VIRULANCE GENES AND VARIATION OF Acinetobacter Baumannii

Özet

Acinetobacter baumannii is gram-negative bacteria associated with hospital-acquired illnesses. The study involved molecular investigation of virulence genes of A. baumannii isolated from different sources using specific primers. Six genes were used for important virulence factors in this bacteria (ompA, plcN, csgA, lasB, iutA, fyuA), partially investigated using PCR, results showed the presence of these genes as 100% and 52.6% and 63.1% and 21% and 21% respectively, while the gene fyu A was not found in any A. baumanni isolate. The study also involved genetic variation in the DNA of these isolates using a universal primer (ERIC2). The results showed that 12 genotypes among 20 isolates of A.baumannii bacteria were revealed. The twelve isolates were divided into four groups, each of which contained genotype-identical isolates linked by bands with comparable molecular weights, as well as (8) single isolates that did not belong to any of the four groups.