Antioxidative Effects of Mash Beans Depending on Gender and High Fat Intake in a Model Organism

Abstract

Objective: In the study, the aim was to to determine the effect of Mash bean, an antioxidant source, against oxidative stress due to gender and high fat intake. Materials and Methods: For this purpose, local and commercial Mash was added to the fatty and non-fatty diet of the model organism (Drosophila melanogaster) which were let to grow. Malondialdehyde and glutathione S-transferase (GST) activities were determined in adult females and males. Results: According to the results, it was determined that Mash bean increased the GST activity of the insect. Feeding with non-commercial mash bean (NC-MB) and high-fat diet decreased lipid peroxidation (LPO) in females, whereas commercial mash bean (C-MB) had opposite effects. Conclusion: It was concluded that the NC-MB seeds were more successful in preventing LPO than C-MB. In our study, the nutrition of male individuals with a fat diet or a non-fat diet did not change the amount of LPO.