Comparative Effect of Agrowastes on Bacterial Cellulose Production by Acinetobacter sp. BAN1 and Acetobacter pasteurianus PW1

Özet

Comparative effect of Pineapple waste medium (PIWAM) and Pawpaw waste medium (PAWAM) on the production of biocellulose (BC) by Acinetobacter sp. BAN1 and Acetobacter pasteurianus PW1 was investigated. The dry weight of the BC produced by Acinetobacter sp. BAN1 ranged from 0.4 – 0.6 g l-1 and 0.2 – 1.1 g l-1 in PIWAM and PAWAM. The dry weight of the BC produced by Acetobacter pasteurianus PW1 ranged from 0.1 – 3.9 g l-1 and 0.2 – 1.0 g l-1 in PIWAM and PAWAM. PIWAM supported the highest BC production by the two strains. 37°C, 35°C and 28°C supported the highest BC production in PIWAM and PAWAM by the isolates. pH 8, pH 3 and pH 7 was the best for BC by Acinetobacter sp. BAN1 and Acetobacter pasteurianus PW1 in PIWAM and PAWAM. FTIR spectrometry analysis of the BC showed the presence of 𝛽- glycosidic bonds connecting the carbohydrate monomers, hydroxyl groups, carbonyl groups and vibrating sugar rings. In conclusion, the study has demonstrated the ability of utilizing low cost agro wastes as substrates for bacterial cellulose production.