Journals / Ulusal Travma Dergisi / 1999 / Cilt: 5 - Sayı: 1
Role of cyclosporine A on organ injury seen after hemorrhagic shock and reperfusion in rats
- Journal
- Ulusal Travma Dergisi
- Pages
- 11–18
- DOI
- —
Abstract
Neutrophils are an importantfactor in the organ injury associated with ischemia and reperfiision (I-R). In experimental conditions, besides many chemicals, immunosuppressive agents like cyclosporine A (CsA) and FK506 were also used to prevent I-R injury. But effect ofimmunosuppressives on neutrophils still remains unclear. We designed an experimental study to investigate the role of CsA in organ injury after hemorrhagic shock and reperfiision in rats. Forty Wistar albino rats were divided into 4 groups. Gl: Control, G2:Shock, G3: Shock+Reperfusion, G4:CsA.Shockwasinducedbywithdrawingof2,5mlbloodviafemoralvein. Theshed blood was then reinfiised after 20 min in reperfiision groups. In G4, CsA (20 mg/kg) was given by a single IVinjectionjust before (5 min) the perjusion. Rats were sacrified24 hours after operation. Plasma malondialdehyde (MDA) levels of all groups and, MDA and myeloperoxidase (MPO) levels of liver, lung, small bowel and kidney were measured. In addition, neutrophil counts were obtained from all organs. After I-R, MDA levels of plasma and all organs increased and then returned to normal by CsA administration. This observations revealed the role of five oxygene radicals in I-R injury. But CsA-induced MPO decrease was obtained in only small bowel. In addition, reduction in neutrophil infiltration by CsA was seen in small bowel and kidney. We concluded that, CsA has a beneficial effect on I-R-induced organ injury. But, at least in liver and lung, these results couldnt attributed to reduced neutrophil infiltration, However, increase of CsA dose may cause widespread reduction in neutrophil counts. gFurther investigations are necessary to clarify the exact role of CsA on I-R injury.
Özet
iskemiye maruz kalan organlarda, reperfiizyon sonrasi goriilen hasann (l-R hasan) ortaya ctkmasmda notrofiller onemli rol oynamaktadir. Notrofil araaligiyla gelisen I-R hasanm onlemek arityayla, deneysel kosullarda, cesitli maddelerin yamsira siklosporin A (CsA) ve FK506gibi immiinosupresifajanlar da kullamimishr. Ancak immunasupresiflerin notrofiller uzerindeki etki mekanizmalart hentiz tarn olarak anlastlamamistir. Hipovolemik sok olusturulan sicanlarda, notrofil araaligiyla olusan I-R hasartna CsA'mn etkilerini arastirmayi amacladik. Qihsmatmzda, her birilO disi Wistar albino sican iceren 4 grup olusturuldu. Gl: Kontrol, G2: §ok, G3: §ok+Reperfiizyon, G4: CsA §ok olusturmak icin, denekletden femoral venyoluylayaklasik2,5 ml kan almdi. Reperfiizyon gruplannda, altnan kan 20 dk sonra ayniyoldan reperfiize edildi. G4'te; reperfiizyondan 5 dk once JV20 mg/kg CsA verildi. Postop 24.cii saatte sakrifiye edilen deneklerde plazmada malonildialdehit (MDA); karaciger, akciger, ince barsak ve bobreklerde ise MDA ve miyeloperoksidaz (MPO) olciimu ve notrofil sayimiyapildi. I-R sonrasi plazma ve turn dokularda artan MDA degerleri, CsA verildikten sonra normale inmistir. Bu bulgular, I-R hasannda serbest oksijen radikallerinin (SOR) rolunu gostermektedir. Ancak, MPO degerleri CsA verilmesi He sadece ince barsakta normale inmistir. CsA'mn notrofil infiltrasyonunu azaltia etkileri deyine ince barsak ve bobrektegorulmiistur. Sonuc olarak, CsA tedavisinin I-R hasanm azaltiayonde etkiye sahip oldugu saptanmistir. Ancak bu etkinin, en azmdan karaciger ve akdgerde, notrofil infiltrasyonunu azaltmak yoluyla olmadtgim dusunmekteyiz. CsA'mn dozu arttinldigt taktirde, bu etki belki turn dokularda yaygmlasabilir. CsA'mn l-R hasarmdaki etki mekanizmasmm tarn olarak anlasilabilmesi icinyeni arastirmalara ihtiyac vardir.