Histological influences of low-dose-morphine hydrochlorur on rat kidneys

Düşük doz morfin hidroklorünün rat böbrekleri üzerindeki histolojik etkisi

Abstract

In this study the effects of low-dose morphine hydrochlorur on rat kidney were determined at light microscopical level. A total of 30 Sprague Dawley rats from both sexes (15 male and 15 female) were used. Five rats from both sexes were served as controls, whereas 10 rats from each sex were used as experimental group. Control animals were injected subcutaneously with physiological saline (5mg/kg), the animals of experimental group were given morphine hydrochlorur at a dose of 5 mg/kg in every other day. At the end of the experiment, the animals were perfused with buffered formaline (pH=7.4) under anaesthesia, and kidneys were extirpated, and morphometrical measurements (thickness, height, length) were taken. Following, kidney samples were processed by means of routine histological techniques and the sections were taken at 7µm thickness, and stained. Slides were then examined using light microscope. The investigations have revealed that the low-dose-morphine hydrochlorur caused to glomerular destruction, epithelial necrosis and desquamation in both proximal and distal tubuli, vascular hyperemia and hemorrhagies. Based on the findings, it was concluded that the results of this study might be specific to the rat kidney which is devoid of detoxification enzymes in tubuler epithelial cells and a study should be performed on an animal having similar metabolism with human.

Özet

Bu çalismada, düsük doz morfin hidroklorür'ün rat böbregi üzerindeki etkileri isik mikroskobik seviyede belirlendi. Çalismada 15'i erkek ve 15'i disi olmak üzere 30 adet Sprague Dawley irki rat kullanildi. Her iki cinsten 5'er adedi kontrol grubu, 10'ar adedi ise deney grubu olarak ayrildi. Kontrol grubu hayvanlara günasiri derialti yoluyla serum fizyolojik (5mg/kg dozunda), deney grubu hayvanlara ise 5 mg/kg dozunda morfin hidroklorür verildi. Deney sonunda hayvanlar genel anestezi altinda tamponlu formolle (pH=7.4) perfüze edildi ve böbrekler çikarilarak morfometrik ölçümleri (en, boy, kalinlik) yapildi. Takiben, böbrek dokusu örneklerinden rutin histolojik tekniklerle preparatlar hazirlanarak 7µm kalinliginda kesitler alindi ve boyandi. Preparatlar isik mikroskobuyla incelendi. Yapilan incelemelerde, düsük dozda verilen morfin hidroklorürün glomerüler yikimlanma, proksimal ve distal tubuluslarda epitel nekrozu ve deskuamasyonuna, damarlarda hiperemi ve hemorajilere neden oldugu tespit edildi. Tespit edilen bozukluklarin, böbrek tubulus epitel hücrelerinde detoksifikasyon enzimlerine sahip olmayan ratlara özgü olabilecegi ve metabolizmasi insana yakin olan bir türde benzer bir çalismanin yapilmasinin yararli olacagi sonucuna varildi.