Journals / Selçuk Tıp Dergisi / 2000 / Cilt: 16 - Sayı: 1
The effect of vitamine E and ursodeoxycholic acid on immune system in obstructive jaundice
- Journal
- Selçuk Tıp Dergisi
- Pages
- 17–25
- DOI
- —
Abstract
Purpose: The aim of this study is to investigate the effect of Vitamine E and Ursodeoxycholic acid (UDCA) on the leucocyte count, the ratio of T, B and Null leymphocyte and translocation of E. coli labilled with flourescein in an experimental obstructive jaundice model in rats. Material and Methods: Seventy male Wistar-albino rats were used in this study. The animals were divided into four groups including 15 rats and obstructive jaundice were performed in all rats. In the first group; obstructive jaundice was performed only, In the second group; 25 mg/kg UDCA was given orally betwene 8-14 days. In the third group; 500 mg/kg/im Vitamine E was given in every two days betwene 8-14 days (three times). In the fourth group Vitamine E and UDCA were given together as mentioned above. In the left ten rats; sham procedure was performed (fifth group). Two ml solution including 109 E. coli labelled with flourescein was given via a naso-gastric tube at 14th day. All rats were sacrificed at 15th day and mesenteric lymphatic nodules (MLN), liver, spleen, small bowel tissue samples and one ml blood samples were taken. Leucocyte types were detected by May-Grüwald-Giemse method and T, B and Null lemphocyte ratio were found bay ANAE enzyme. Serum Bilirubine, SGOT, SGT and Alkaline phosphatase levels were measured. Translocated E. coli number detected in bowell wall. MLN, liver, spleen and blood samples by flourescein microscopy. Findings: There was a significant decrease of lemphocyte ratio in group I and II (p<0.05). The lemphocyte ratio of groups II and III was similar with control group. T and B lemphocyte ratio decreased in all waundiced groups. The decrease was significant in third and fourth groups (p<0.05). Null lemphocyte ratio increased in all groups, but the differences was significant in groups III and IV (p<0.05). E. coli translocation was significant in group I, and there was differences in other groups. Conclusion: Vitamine E caused Null lemphocyte proliferation. UDCA has no direct effect on immune system, but it prevented the lebelled E. coli translocation.
Özet
Bu çalismada tikanma sariligi olusturan siçanlarda; UDKA ve vitamin E'nin lökosit sayisi, T, B ve null lenfosit oranlari ve floresans boya ile isaretli E. coli translokasyonu üzerine etkilerinin arastirilmasi amaçlandi. Gereç ve Yöntem: Arastirmada 70 Wistar-Albino cinsi erkek siçan kullanildi. Tikanma sariligi olusturulan siçanlar 15'erli dört gruba ayrildi. Birinci grup sadece tikanma sariligi olusturulan gruptu. Ikinci gruba 8-14. Günler arasi 25 mg/kg oral UDKA verildi. Üçüncü grup ayni günler arasinda bir haftalik süre içinde günasiri 500 mg/kg intramüsküler (toplam 3 kez) vitamin E verilen deney grubuydu. Dördüncü gruba UDKA ve vitamin E, yukarida belirtilen süre ve dozda birlikte verildi. Geriye kalan 10 denekte (5. Grup) sham islemi uygulandi. 14. Günde tüm siçanlara ml'sinde 109 bakteri içeren floresans boya ile isaretli E.coli 2 ml olarak nazo-gastrik tüp ile verildi. Bir gün sonra steril sartlarda sakrifiye edilen siçanlardan, histopatolojik inceleme için MLN, karaciger, dalak, ince barsak ve kan örnekleri alindi. Kan yaymalarinda May-Grünwald-Giemsa yöntemi ile lökosit formülü, ANAE enzimi ile T, B ve null lenfosit oranlari saptandi. Billirübin, SGOT, SGPT ve alkalen fosfataz çalisildi. Barsak duvari, MLN karaciger, kan ve dalakta floresans mikroskopta isaretli E.coli'ler belirlendi. Bulgular: Lökosit formülünde 1 ve 2. Grupta lenfosit oranlarinda azalma vradi (p<0.05). E vitamini verilen gruplarda ise lenfosit oranlar isham grubu ile benzerdi. T ve B lenfosit oranlari ise tüm tikanma sarilikli gruplarda azalmisti. Bu azalma 3 ve 4. Gruplarda daha belirgindi (p<0.05). Null lenfositlerin orani ise tüm tikanma sarilikli gruplarda artmis, E vitamini verilen gruplarda ise daha belirgin olarak artmisti (p<0.05). Isaretli E. coli'lerin birinci grupta anlamli yükseklik gösterdigi,diger gruplar arasinda fark olmadigi belirlendi. Sonuç: Tikanma sariliklarinda E vitaminini lenfoproliferatif etkiyle Null lenfositlerde anlamli bir artis sagladigi, UDKA'in immün sistemüzerine direkt bir etkisinin olmadigi, ancak isaretli E. colilerin dokulara geçisini E vitaminine esdeger oranda azalttigi tesbit edilmistir.