Journals / Selçuk Tıp Dergisi / 2001 / Cilt: 17 - Sayı: 2
Proliferating cell nuclear antigen (PCNA) in thyroid tumors
- Journal
- Selçuk Tıp Dergisi
- Pages
- 87–90
- DOI
- —
Abstract
We have studied on 47 thyroidectomy material that were came to Firat University Medical Faculty Department of Pathology for the determination of proliferative activity. PCNA method were applied, normal thyroid(9), adenomatous goiter(11), follicular adenoma(7), papillary carcinoma(16), follicular carcinoma(2) and anaplastic carcinoma(2). All cases paraffin sections stained PCNA/cyclin monoclonal antibody. Positive painted nuclei was evaluated and PCNA indices were calculated as percentage. PCNA indices were evaluated normal thyroid %0.5, adenomatous goiter %2.1, follicular adenoma %2.6, papillary carcinoma %6.2, follicular carcinoma %15.7 and anaplastic carcinoma %32.2. As a conclution, thyroid carcinomas showed significantly increased numbers of PCNA positive cells when compared with benign thyroid tumors.
Özet
Bu çalismada tiroid tümörlerinde proliferatif aktivitenin gösterilmesi amaciyla Firat Üniversitesi Tip Fakültesi Patoloji Anabilim Dali'na gelen 47 adet tiroidektomi materyali çalismaya alindi. Normal tiroid (9), nodüler guatr (11), folliküler adenom (7), papiller karsinom (16), folliküler karsinom (2) ve anaplastik karsinom (2)'a immünohistokimyasal olarak PCNA yöntemi uygulandi. Parafine gömülen dokulardan hazirlanan kesitler PCNA/cyclin monoklonal antikorlari ile boyandi. Pozitif boyanan nüveler degerlendirmeye alindi ve yüzde olarak PCNA indeksleri hesaplandi. PCNA indeksleri normal tiroid dokusunda % 0.5, nodüler guatrda % 2.1, folliküler adenomda % 2.6, papiller karsinomda % 6.2, folliküler karsinomda % 15.7 ve anaplastik karsinomda % 32.2 olarak degerlendirildi. Sonuç olarak benign tiroid tümörleri ile malign tiroid tümörleri karsilastirildiginda PCNA indeksinin malign tiroid tümörlerinde belirgin artmis oldugu tesbit edildi.