Isolation and molecular identification of extreme halophilic archaea from Camalti Saltern

Çamaltı tuzlasında ekstrem halofilik archaea izolasyonu ve moleküler karakterizasyonu

Abstract

Isolation and molecular identification of extreme halophilic archaea from Çamaltı Saltern. Extreme halophilic microorganisms are organisms adapted to live at high salt concentrations and therefore have some interests at biotechnological area. In this study, halophilic archaea were isolated from samples taken from the Çamaltı Saltern and identification of isolates were performed with their biochemical tests and 16S rDNA sequencing. Isolates 1, 4, 6, 7, 10, 11, 13 were identified as Haloferax alexandrinus with 99% sequence similarity; isolate 5 was identified as Haloferax alexandrinus with 98% sequence similarity; isolate 12 was identified as Haloferax alexandrinus with 96% sequence similarity; isolate 3 was identified as Haloferax sp. HSC4 with 99% sequence similarity; isolate 8 was identified as Haloferax sp. YT 228 with 97% sequence similarity; isolate 2 was identified as Halobacterium salinarum R1 strain with 99% sequence similarity; 9 isolate was identified as Halobacterium salinarum with 97 %sequence similarity.

Özet

Ekstrem halofilik mikroorganizmalar yüksek tuz koşullarında yaşamaya adapte olmuş bu nedenle biyoteknoloji alanında ilgi gören canlılardır. Bu araştırmada İzmir Çamaltı Tuzlası’ndan alınan örneklerden ekstrem halofilik archaea izolasyonu gerçekleştirilerek bu mikroorganizmaların biyokimyasal testlerinin yanı sıra moleküler karakterizasyonları yapılmıştır. 16S rDNA analizleri sonucu 1, 4, 6, 7, 10, 11, 13 nolu izolatlar %99 oranında Haloferax alexandrinus, 5 nolu izolat %98 oranında Haloferax alexandrinus, 12 nolu izolat %96 benzerlik oranıyla Haloferax alexandrinus, 3 nolu izolat %99 Haloferax sp. HSC4, 8 nolu izolat %97 Haloferax YT 228, 2 nolu izolat %99 Halobacterium salinarum R1 strain, 9 nolu izolat %97 Halobacterium salinarum olarak tanılanmıştır.