Effect of gabapentin on intraocular oxidative stress after spinal cord ischemia-reperfusion injury

Gabapentinin spinal kord iskemi-reperfüzyon yaralanması sonrası intraoküler oksidatif strese etkisi

Abstract

Purpose: In this study, we aim to research the effect of gabapentin on intraocular oxidative stress after spinal cord ischemiareperfusion injury. Materials and Methods: Twenty-four adult, male, New Zealand rabbits were divided into 4 groups; control (n=6), ischemia (n=6), low dose gabapentin (30 mg/kg), (n=6) and high dose gabapentin (200 mg/kg), (n=6) treatment groups. In ischemia and treatment groups, the abdominal aorta was clamped for 30 minutes by an aneurysm clip, approximately 1 cm below the renal artery and 1 cm above the iliac bifurcation. In the control group, only laparotomy was performed in the control group. The animals were killed 48 h later. Vitreous samples were collected for analysis. Levels of tumor necrosis factor-α, malondialdehyde, and total antioxidant status were analyzed as markers of oxidative stress. Results: Oxidative stress is observed to increase in vitreous following spinal cord ischemia-reperfusion injury. In ischemia group the vitreous malondialdehyde and tumor necrosis factor-α levels were significantly higher (p<0.05) and total antioxidant status level was significantly lower compared to the conrol group (p<0.05). The vitreous malondialdehyde and tumor necrosis factor-α levels were significantly lower (p<0.05) and total antioxidant status significantly higher in low and high dose gabapentin groups compared to the ischemia group (p<0.05). When low and high dose gabapentin groups compared with control group, no significant difference were found between vitreous malondialdehyde, tumor necrosis factor-α, and total antioxidant status levels (p>0.05). Conclusions: The experiments conducted in the present study show that gabapentin has a significant effects on lowering the oxidative stress in the eyes following a spinal cord ischemia-reperfusion injury.

Özet

Amaç: Bu çalışmada gabapentinin, spinal kord iskemi-reperfüzyon yaralanması sonrası göz içi oksidatif strese etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Yirmi dört adet Yeni Zelanda erkek tavşan 4 gruba ayrıldı; iskemi (n=6), kontrol (n=6), düşük doz gabapentin (30 mg/kg) (n=6) ve yüksek doz gabapentin (200 mg/kg) (n=6) tedavi grupları. İskemi ve tedavi gruplarında abdominal aorta 30 dakika anevrizma klempi ile renal arterin 1 cm altından ve iliac bifukasyonun 1 cm üstünden klempe edildi. Kontrol grubuna yalnız laparotomi yapıldı. Hayvanlar 48 saat sonra sakrifiye edildi. Vitreus örnekleri analiz için alındı. Tümör nekroz faktör-α, oksidatif stres belirleyicileri olan malondialdehit ve total antioksidan statü düzeyleri ölçüldü. Bulgular: Vitreusta oksidatif stres spinal kord iskemi-reperfüzyon yaralanması sonrasında artmaktadır. İskemi grubu ile kontrol grubu karşılaştırıldığında, iskemi grubunun vitreus malondialdehit ve tümör nekroz faktör-α düzeyleri anlamlı olarak artarken (p 0.05). Sonuç: Gabapentinin spinal kord iskemi-reperfüzyon yaralanması sonrası oluşan oküler oksidatif stresi azaltmada belirgin etkisi vardır.