Journals / EurAsian Journal of BioSciences (elektronik) / 2009 / Sayı: 3
Hymenocrater calycinus özütünün fitokimyası ve antimikrobiyal bileşikleri
- Issue
- 2009 · Sayı: 3
- Pages
- 64–68
- DOI
- —
Abstract
The genus, Hymenocrater, belongs to the plant family Lamiaceae which contains eleven shrub species, of which H. calycinus belonges and grows wildly in Northeastern Iran. From the ethyl acetate and methanol extracts of the flowered aerial parts of Hymenocrater calycinus, four compounds were isolated using chromatographic methods and identified by spectroscopic data (MS, 1H- and 13C-NMR, HMQC, HMBC and 1H-1H COSY). The effect of rosmarinic acid, as the main component in our study was applied to Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger by the broth dilution method. Isolated compounds were identified as ß-sitosterol (1), ursolic acid (2), rosmarinic acid (3) and quercetin-3-O- rutinoside (4). The results of our assay against bacteria and fungi show that, rosmarinic acid has an antifungal property against Candida albicans (MIC, 250 μg mL-1).
Özet
Hymenocrater cinsi, Lamiaceae familyasina dahil olup, onbir çali türünü barindirir. Bunlarin içinden H. calycinus kuzeydogu Iran'da dogal olarak yetisir. Hymenocrater calycinus'un çiçekli kisimlarindan elde edilen etil asetat ve metanol özütlerinden, kromatografik yöntemlerle dört bilesik izole edildi vespektroskopik data araciligi ile tanimlandilar (MS, 1H- ve 13C-NMR, HMQC, HMBC ve 1H-1H COSY). Çalismamizin ana bilesigi olan rozmarinik asitin etkisi, broth seyreltme metodu kullanilarak, Staphylococcus aureus, Escherichia coli, Candida albicans ve Aspergillus niger'a uygulandi. Izole edilen bilesenler; ß-sitosterol (1), ursolic acid (2), rosmarinic acid (3) ve quercetin-3-O- rutinoside (4) seklinde belirlendi. Bakteri ve mantarlarla yaptigimiz çalisma, rozmarinik asitin Candida albicans (MIC, 250 μg mL-1)'a karsi antifungal bir etkisi oldugunu göstermektedir.