Simultaneous determination of selected flavonoids from different Cistusspecies by HPLC-PDA

Özet

A rapid and simple high-performance liquid chromatography method with a Photo Diode Array (PDA) detector is developed for the simultaneous analysis of seven kaempferol, quercetin and myricetin derivatives along with an acylated flavonoid glycoside; trans-tiliroside from different Cistusspecies. In this study, the qualitative and quantitative analysis of the methanolic extracts of three Cistusspecies (C. creticusL., C. laurifoliusL. and C. salviifoliusL.) growing in Anatolia in terms of characterization of flavonoid compounds were performed by RP-HPLC for the first time. Trans-tiliroside, a mono-coumaroyl kaempferol glucoside was found to be the most abundant flavonoid in C. salviifolius(0.276±0.003 g/100 gextract) and C. creticus(0.253±0.001 g/100 gextract) while hyperin (0.149±0.004 g/100 gextract) and myricetin 3-O-β-galactopyranoside (0.139±0.006 g/100 gextract) were found to be the most intense flavonoids in C. laurifoliussamples.The described HPLC method appears suitable for the determination of the Cistusflavonols and their glycosides and can be considered as an effective and alternative procedure for the identification and quantification of this important class of biologically active compounds.