Journals / Medical Network Klinik Bilimler ve Doktor / 2003 / Cilt: 9 - Sayı: 1
Methylene blue in the treatment of experimental colitis
- Pages
- 44–48
- DOI
- —
Abstract
Objective: Research of drugs for the treatment of inflammatory bowel disease is ongoing. The effect of methylene blue was investigated in the treatment of experimental colitis. Material and Methods: In Gülhane Military Medical Academy Research Center, 80 Sprague -Dawley rats, in which experimental colitis was produced by administering 1.5 ml 6% acetic acid by rectal route, were divided into two equal groups (Group A and B). Beginning from thirty minutes after the administration of acetic acid and in every 12 hours throughout the study period, 1.5 ml 0.09% NaCl and 1.5 ml 1% methylene blue were applied in group A and B, respectively. A segment of distal colon, 5 cm in length, was resected in 10 rats in each group at day 3, 7, 10 and 14 after acetic acid instillation, and then histological scoring was performed. The histological scores were analyzed by t-test for independent samples between groups, while Mann Whitney U test was used to compare the death ratio in each group. P value less than 0.05 was accepted statistically significant. Results: Although death rate for methylene blue group was lower than control group, it was not statistically significant between groups (p>0.05). The average histological scores in Group A and B were 2.18±0.73 and 0.34± 0.53 respectively; it was statistically significant between groups (p<0.0001). Conclusion: It is found that methylene blue is effective in the treatment of acetic acid induced colitis by topical application. It is thought that it might be used in conjunction with the other treatment protocols because of its different mechanism.
Özet
Amaç: İnflamatuar barsak hastalığının tedavisi için ilaç araştırmaları devam etmektedir. Deneysel kolit tedavisinde metilen mavisinin etkisi araştırıldı. Gereç ve Yöntem: GATA Araştırma Merkezinde rektal yoldan 1.5 ml %6'lık asetik asit verilerek deneysel kolit oluşturulan Sprague-Dawley cinsi 80 rat iki eşit gruba (Grup A ve B) ayrıldı. Asetik asit verilmesinden 30 dakika sonra başlayıp çalışma süresi boyunca 12 saatte bir kez Grup A 'da (kontrol grubu) 1.5 ml serum fizyolojik; Grup B' de 1.5 ml %1'lik metilen mavisi verildi. Uygulamadan 3, 7, 10 ve 14 gün sonra her gruptan 10 ratta distal kolondan 5 cm'lik kısım rezeke edilerek histolojik skorlama yapıldı. Mann-Whitney U testi her grupta ölüm oranını karşılaştırmada kullanılırken, gruplar arasında histolojik skorlar bağımsız örneklerde t- testi ile değerlendirildi. p 0.05). Histolojik skor ortalaması Grup A ve B'de sırasıyla 2.18±0.73 ve 0.34±0.53 olup, gruplar arasında istatistiksel olarak anlamlı fark bulundu (p<0.0001). Sonuç: Metilen mavisi lokal uygulamada asetik asitle oluşturulan kolitin tedavisinde etkili bulunmuştur. Farklı etki mekanizması nedeniyle, diğer ilaç tedavi protokolleriyle beraber kullanılabileceği düşünülmektedir.