P16 staining patterns and their relationships with clinical parameters in cases of cervical intraepithelial neoplasia

Özet

The aim of this study is to investigate the contribution of the p16 immunohistochemical marker in the cervical intraepithelial neoplasia (CIN) cases in the distinction between CIN1, CIN2, CIN3 and the relationship of CINs with age, localization. CIN1, CIN2, CIN3 preparations of 2015 in the pathology department were retrospectively analyzed. New sections of 3 μm thickness were obtained on polylysine slides from paraffin blocks of these tissues. Samples with immunohistochemical staining with p16 were evaluated under a light microscope.p16 expression was semiquantitatively assessed according to staining intensity with 4 staining levels as score 0,1,2,3. As a result of the Kruskal-Wallis test, a significant difference was found between the scores in the CIN groups (p 0.05). The variations between CIN groups and anatomical locations on the cervix were examined with Chi-square test. There was no change in the frequency distribution of CIN groups in the anatomical locations on the cervix (p> 0.05). In cervical intraepithelial neoplasms, p16 was found to show increased staining with the severity of the lesion. It was observed that p16 expression is important in CIN evaluation and reduces subjective evaluation.