Changes in thiol/disulfide homeostasis in patients with chronic kidney disease

Özet

Thiol/disulfide homeostasis (TDH) is a new marker of oxidative stress. In this study, we would like to determine the changes in TDH in hemodialysis (HD) patients with chronic kidney disease (CKD). This cross-sectional study was conducted in the Nephrology Clinic of Konya Training and Research Hospital. A total of 197 individuals including 75 HD patients, 41 end stage renal disease (ESRD) patients (having stage 3-5 CKD but not receiving hemodialysis), and 81 healthy controls were enrolled in the study. Serum native thiol, total thiol, and disulfide levels were measured with a new method developed by Erel and Neselioglu. It was determined that there was a statistically significant difference in the mean age, body mass index (BMI), modification of diet in renal disease (MDRD), and creatinine level between the three groups (p<0.001). It was shown that there was a statistically significant difference in native thiol and total thiol levels and disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios between the three groups, whereas there was no statistically significant difference in disulfide level between the three groups. Consequently, our study found that TDH shifted more towards the left (disulfide side) and oxidative stress was higher in both HD (markedly) and CKD groups when compared to the control group.