The Effect of Some Plant Hormones on In Vitro Properties of Sainfoin (Onobrychis sativa L.)

Bazı Bitki Hormonlarının Korungada (Onobrychis sativa L.) In Vitro Özellikler Üzerine Etkisi

Abstract

This study prepared was carried out determining plant hormones (PH) (NAA, BAP, KIN and TDZ)on formation of tissue culture on the sainfoin plants (Onobrychis sativa L.) grown in in-vitro conditions. This studywhich is applied 42 different methods was performed. In the first stage, the seeds were sprouted in hormone-freeenvironment. In the second stage, the explants (cotyledon, root and hypocotyl) obtained from the plantlets weretransferred to the mediums including different hormone, dose and hormone-dose combinations. For the effect ofplant growth hormones, the highest number of shoots (29.0 nr) was obtained from root explant the dose 1.5 mg l-1 ofBAP in the end of 90th day. Shoot number on cotyledones increased depending on incereasing number of treatment/day and the highest number of shoot (7.8 nr) was obtained from the dose 1.0 mg l-1 of TDZ in the end of 90th day.The best result in terms of rooting (14.8 nr) was obtained from the dose 0.4 mg l-1 of NAA from hypocotyl.

Özet

Bu çalışma, bitki hormonlarından (BH) naftalen asetik asit (NAA), 6-benzil amino pürin (BAP), kinetin