The Effects of Amoxicillin and Vancomycin Hydrochloride Hydrate on Glutathione S-Transferase Enzyme Activity: An in vitro study

Glutatyon S-Transferaz Enzim Aktivitesi Üzerine Amoksilin ve Vankomisin Hidroklorid Hidratın Etkisi: Bir in vitro çalışma

Abstract

Glutathione S-transferase (GST) enzymes detoxify a broad spectrum of xenobiotics, includingchemotherapeutic drugs, environmental carcinogens, and endogenous molecules. Glutathione transferases catalyzethe conversion of some metabolites into less toxic substances. Phase II reactions, which often result in detoxification,are conjugation reactions of glutathione through GST enzymes that protect the organism from the attacks of highlyreactive electrophilic substances. Since the GST enzyme can metabolize toxic exogenous compounds, it has beenextensively investigated in mammals. In this study, the GST enzyme was purified in human erythrocytes with aspecific activity of 5.56 EU mg-1 protein and a yield of 2316.0 fold with 65% efficienc. SDS-polyacrylamide gelelectrophoresis was performed to check the purity of the purified enzyme and the molecular mass was determinedto be about 26 kDa. The IC50 and Ki values for amoxycillin and vancomycin hydrochloride drugs on the purifiedGST enzyme were calculated and the type of inhibition was determined. As a result of the inhibition studies, weobserved that these antibiotics inhibited the GST enzyme at low doses.

Özet

Glutatyon S-transferaz enzimleri kemoterapik ilaçlar, çevresel karsinojenler, endojen moleküller olmak