Journals / Hacettepe Journal of Biology and Chemistry / 2011 / Cilt: 39 - Sayı: 2
Activatory Effects of Oxytetracycline on Some α-Carbonic Anhydrases
- Pages
- 207–212
- DOI
- —
Abstract
Three -carbonic anhydrases, including human carbonic anhydrase I and II hCA I, II and sheep kidney carbonic anhydrase sCA were purified and kinetic interactions between the enzymes and oxytetracycline were investigated. The purification procedure was composed of preparation of homogenate or hemolysate and affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. Oxytetracycline exhibited in vitro activatory effects on hCA I, II and sCA enzymes activity. Activity % values for this antibiotic were determined by plotting activity % vs. [A]
Özet
Üç alfa-karbonik anhidraz, insan karbonik anhidraz I, II hCA I, II ve koyun böbrek karbonik anhidraz sCA saflaştırıldı ve enzimlerle oksitetrasiklinin kinetik etkileşmeleri incelendi. Saflaştırma prosedürü homojenat hazırlama ve Sepharose 4B-trizonsüllfanilamid afinite kramatografisinden oluştu. Oksitetrasiklin hCA I, II ve sCA enzim aktivitelerinde in vitro aktivatör etkisi gösterdi. Bu antibiyotik için % aktivite-[A] grafiği kullanılarak % aktivite değerleri belirlendi