Journals / Turkish Journal of Veterinary and Animal Sciences / 2001 / Cilt: 25 - Sayı: 6
Detection of Antibodies Against Salmonella Enteritidis in Chicken Serum and Egg Yolk with ELISA
- Pages
- 983–988
- DOI
- —
Abstract
A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. Forty-one (23.7%) of the ELISA positive sera were also positive in the rapid plate agglutination test.
Özet
A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. Forty-one (23.7%) of the ELISA positive sera were also positive in the rapid plate agglutination test.