DETERMINATION OF PHENOLIC SUBSTANCE AND ANTIOXIDANT ACTIVITY IN SOME TREE BARK EXTRACTS

Abstract

In this study, the total amount of phenolic substances and antioxidant activity were determined in the extracts obtained from tree barks of 3 coniferious (scots pine, spruce, cedar) and 8 deciduous (poplar, chestnut, oak, pseudoacacia, beech, eucalyptus, iron and mimosa). The tree barks were extracted with alcohol: benzene (1: 2 v / v) solvent according to TAPPI T 204 cm-07 standard. The antioxidant activities (AA) of the extracts were determined by the capture activity of 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical. The total amount of phenol in the contents of the extracts (TPM (mg-GAE / g-DL)) was determined by the Folin-Ciocalteu method. It also provides information about the antioxidant activity of the extract due to its total phenolic substance dependence on oxidation-reduction reactions and the total amount of phenolic substance in the bark extract has a linear relationship between TPM (mg-GAE / g-DL) and antioxidant activity (AA). Compared to coniferous tree barks; higher tannin content and antioxidant activity were determined in the leafy (excluding beech) bark extracts. The highest TPM (mg-GAE / g-DL) and AA were found especially in the extracts of mimosa (Acacia Dealbata L.), iron (Casuarina equisetifolia L.), oak (Quercus pontica L.) and poplar (Populus tremula L.) species.