Journals / Turkish Journal of Botany / 1999 / Cilt: 23 - Sayı: 5
Regeneration and Agrobacterium-mediated Transformation Studies in Tomato ( Lycopersicon esculentum Miller)
- Journal
- Turkish Journal of Botany
- Pages
- 345–348
- DOI
- —
Abstract
An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtain rooted plants from leaf pieces of two tomato cul-tivars (ES58 and WC156) within 3-4 months. Here, we also present our preliminary results on transformation experiments carried out with 8-day-old cotyledon explants and Agrobacterium tumefaciensEHA105 strain.
Özet
An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtain rooted plants from leaf pieces of two tomato cul-tivars (ES58 and WC156) within 3-4 months. Here, we also present our preliminary results on transformation experiments carried out with 8-day-old cotyledon explants and Agrobacterium tumefaciensEHA105 strain.