entrCYTOTOXIC AND APOPTOTIC EFFECTS OF NOVEL SYNTHESIZED FERULIC ACID DERIVATIVES ON LEUKEMIA CELL LINESCYTOTOXIC AND APOPTOTIC EFFECTS OF NOVEL SYNTHESIZED FERULIC ACID DERIVATIVES ON LEUKEMIA CELL LINES

Abstract

Ferulic acid (FA), or 4-hydroxy-3-methoxycinnamic acid, is a phenolic compound that can be used in many pharmacological applications, especially in the area of cancer. In this study, four new FA-quinoline esters were successfully synthesized and characterized using 1H-NMR, 13C-NMR, HR-Mass, Elemental Analysis, and FT-IR techniques. Synthesized compounds were tested on leukemia cell lines (HL-60 and K562) with WST-1 assay and Annexin V-PI assay via flow cytometry. The WST-1 assay of compounds in HL-60 and K562 leukemia cells yielded IC50 124.4 and 205.4 μM/mL, respectively. According to the results, both the cytotoxic effect and apoptotic effects of 2c increased on HL-60 cell.