The Effects of Edaravone on Experimental Brain Ischemia/Reperfusion Injury in Rats

Özet

Introduction: Millions of people worldwide die or are disabled every year due to acute ischemic stroke. In this study, our aim was to demonstrate the neuroprotective effects of Edaravone in experimental cerebral ischemia/reperfusion injury in rats. Methods: Twenty-eight female Sprague Dawley rats were divided into four main groups. The ischemia group underwent bilateral carotid artery clipping and hypotension for 30 min. The Edavarone group received intraperitoneal 30 mg/kg/day Edavarone following 30-min bilateral carotid artery clipping+hypotension. Skin laceration only was applied to the control group, and anesthesia only was applied for 15 min to the anesthesia group. At the end of the 10th day, all rats were sacrificed, and blood samples and brain tissues were collected for analysis. Results: Ischemia group malondialdehyde (MDA) values were significantly higher than those of the control and Edaravone groups. Edaravone group tissue MDA values (258±13.8 nmol/g) were significantly lower than those of the ischemia group (368±36.6 nmol/g) (p<0.05). Edaravone group tissue total oxidant status (TOS) was significantly lower than in the anesthesia and ischemia groups (96.9±13.4 mmol H2O2 Eq/L (g)). A statistically significant difference was observed between the groups’ serum TOS values (p<0.05). Discussion and Conclusion: This study shows that Edaravone reduced or prevented ischemic damage in rat brain tissue by causing significant changes in oxidant and antioxidant parameters.