Journals / Turkish Journal of Biology / 2010 / Cilt: 34 - Sayı: 1
Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells
- Journal
- Turkish Journal of Biology
- Pages
- 25–34
- DOI
- —
Abstract
The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that the CH2Cl2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible anti-inflammatory candidates for human health.
Özet
The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that the CH2Cl2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible anti-inflammatory candidates for human health.