Journals / Turkish Journal of Biology / 2012 / Cilt: 36 - Sayı: 1
Protective effects of b-carotene and silymarin on human lymphocytes
- Journal
- Turkish Journal of Biology
- Pages
- 47–52
- DOI
- —
Abstract
Beta-carotene and silymarin have antioxidant properties against oxidative damage and are used as dietary supplements. The aim of this study was to assess the protective effects of b-carotene and silymarin on healthy human lymphocytes exposed to L-arginine-induced oxidative damage. Study samples were lymphocyte cultures set up from venous blood obtained from 6 healthy individuals (3 males and 3 females). Oxidative DNA damage was induced by L-arginine. b-Carotene and silymarin were added to the cultures as antioxidants to observe their protective effects, if any. Alkaline single-cell gel electrophoresis (SCGE) was used to evaluate DNA damage. While L-arginine generated genotoxic damage in the lymphocyte cultures, both b-carotene and silymarin had positive effects on the reversal of the induced genotoxicity. When b-carotene was applied in combination with silymarin, there was no significant change in mean tail moments in comparison to the use of b-carotene or silymarin alone. The data presented here provide a comparative, preliminary insight into the protective role of both b-carotene and silymarin on L-arginine-induced genotoxicity in in vitro lymphocyte cultures.
Özet
Beta-carotene and silymarin have antioxidant properties against oxidative damage and are used as dietary supplements. The aim of this study was to assess the protective effects of b-carotene and silymarin on healthy human lymphocytes exposed to L-arginine-induced oxidative damage. Study samples were lymphocyte cultures set up from venous blood obtained from 6 healthy individuals (3 males and 3 females). Oxidative DNA damage was induced by L-arginine. b-Carotene and silymarin were added to the cultures as antioxidants to observe their protective effects, if any. Alkaline single-cell gel electrophoresis (SCGE) was used to evaluate DNA damage. While L-arginine generated genotoxic damage in the lymphocyte cultures, both b-carotene and silymarin had positive effects on the reversal of the induced genotoxicity. When b-carotene was applied in combination with silymarin, there was no significant change in mean tail moments in comparison to the use of b-carotene or silymarin alone. The data presented here provide a comparative, preliminary insight into the protective role of both b-carotene and silymarin on L-arginine-induced genotoxicity in in vitro lymphocyte cultures.