Journals / Turkish Journal of Veterinary and Animal Sciences / 2002 / Cilt: 26 - Sayı: 2
Comparing the Birth Rates of Microinjected Hybrid Mouse Zygotes Transferred into Outbred and Hybrid Foster Mothers
- Pages
- 329–333
- DOI
- —
Abstract
The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P
Özet
The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (P