Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Abstract

Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malign phenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bone marrow-derived mesenchymal stem cells BM-MSCs to have this ability due to cell-cell interaction, released cytokines, and organization with the extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character.Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in 3-dimensional 3D in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3D structure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects. The invasion and migration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independent samples. All P-values