Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1977 / Cilt: 4 - Sayı: 11-12
Comparative titration test of sheep pox virus produced by Borrel method on sheep and sheep kidney tissue culture.
- Pages
- 112–128
- DOI
- —
Abstract
This study was conducted to compare th e titration of sheep pox virus produced by Borrel method on sheep and sheep kidney tissue culture. 8 dilutions of the virus with 1/10.000 to 1/1.280.000 were used for titrations. Results in sheep were appaered acrording to the pustules and dose minimal reaction (DMR) after intadermicly injections of the vaccine virus dilutions. Any sheep refractory in the exper~ment were disc?trded. 21 .sheep were used in the study and they were kıvırcık race which were more susceptible to the virus. Results in the sheep kidney tissue culture were fallowed · for cytopathic efect (CPE) under microscope for 12 days. CPE were appaered by the method of Reed and Muench and explained by tissue culture infective dose (TCID/50). · Titrations on sheep were estımated as being with a range of 10-5,80 - 10-6,40 where as an tissue culture 10-5,65 10-6,35 the results of the both virus titrations were similar to each other, but titrations of virus on sheep kidney tissue culture were obviosly more economic and practical than the regular method with pustules on sheep.
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