Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1979 / Cilt: 5 - Sayı: 1-2-3
Studies on the preparation and antigenicity of the vaccine against leptospirosis.
- Pages
- 44–58
- DOI
- —
Abstract
The vaccine was prepared fröm Leptospira grippotyphosa Haymana strain which was isolated by the author from Haymana- Ankara. The culture was grawn in Chang's medium supplemented with vitamin Bıı for 9 days. Formelin (37 %) was added in the ratio of 0,05 % after harvesting the culture. The number of the leptospira organisms were counted with a haemocytometer and the culture was filtered through types SM, 11305-0,65 pore size sartari us membrane. Leptospira organisms on the filter membrane we.re collected in to a steril flask at concentration of 1,5 x 109 /ml. with saline solution containing 0,01 % merthiolate·. The monovalent vaccine was produced from Haymana-strain. CL. grippotyphosa) purity of the vaccine was ascertained in nutrient broth, blood agar (aerobic and anaerobic). Fletcher's agar, Chang medium, Littman Oxgall agar, mycobiotic agar and Sabourod's medium. Safety of the vaccine was checkedin goats, rabbits, guinea pigs and hamster.s InnocuÜy of the vaccine was alsa checked by inoculating two guinea pigs with 5 ml. of the vaccine. For untowards reaction vaccinated animals were obserwed for a period of 21 days. At 21 post vaccination day blood samples from the tes animals were tested by micro agglutination Lysis test. and titres were as follows: Two goats 1/100, one· rabbit 1/200, two 1/800, two 1/1600 and guineo-pigs 1/100- 1/800. The efficeacy of the vaccine w as tes te d by chaJlenging. vaccinated and 12 control hamsters at 21 post vaccination day with a virulent strain of L. grippotyphosa. All of the control hamsters died within 14 days wheras the vaccinated animals survived.
Özet
Bu çalışma Ülkemizde u~un zamandır eksikliği duyulan leptospirosis hastalığına karşı bir aşı hazırlamak için yapıldı. Aşımızda Ankara- Haymadan izole ettiğimiz, patojen L. grippotyphosa sero grubundan yerel haymana suşu kullanıldı. Aşı üretimi Bıı vitamini katılmış Chang vasatında yapıldı. Üretimden sonra % 0,05 formone inaktive edildi. Aşı süzüldükten sonra 1,5 x 10-ıı germ olacak şekilde % 0,01 lik merthioletli fizyolojik tuzlu su ile sulandırıldı. Aşı mono:.. valan, formolle· inaktive edilmiş bir aşıdır. Sterilite kontrolunda aşınan saf ve temiz oJduğu saptandı. Bağışıklık testlerinden M.A testi ile keçi, tavşan, kabaylatın kan serumları işlendi. Yeterli seviyede immun antikdrlar bulundu. Aynı hayvanlarda aktif protektif test uygulandı. % 69 -% 84,5 arasında bağışıklık sağlandı.