In Vitro Anticandidal and Antibiofilm Activities of Capsella bursa pastoris Root Against Candida Species

Abstract

This research displays the antimicrobial and antibiofilm activities of Capsella bursa-pastoris methanol and ethanol extracts against Candida species (C. albicans, C. dupliniensis, C. parapsilosis, C. glabrata and C. tropicalis). Methanol and ethanol extracts of Capsella bursa-pastoris were obtained by maceration method. The anticandidal activity of extracts was estimated by agar well diffusion and microdilution assays. The antibiofilm activity of extracts was determined with crystal violet assay. The root extract of Capsella bursa pastoris has an anticandidal activity for all Candida species. The flower extract of this plant has the anticandidal activity of C. albicans. Microdilution assays showed that plant extract was recorded as >250 mg/L. However, CV assay shows that increasing the concentration of root extracts significantly reduced biofilm formation. The minimum biofilm inhibitor concentration value against C. tropicalis was recorded as 64 mg/L. These results suggest that Capsella bursa-pastoris root extracts can be used as antibiofilm agents against Candida species.