THE GENOTOXICITY AND CYTOTOXICITY OF FUNGUCITE (LUNA EXPERIENCE-EC 400) FLUOPYRAM/TEBACONAZOL COMBINATION SINGLE GEL CELL ELECTROPHORESIS (COMET ASSAY) AND MICRONUCLEUS ASSAY IN RAT BONE MARROW

Abstract

Pesticides are chemical substances that prevent from harmful organisms and reduce their harms. Pesticides areclassified by their durability time, chemical structure and organisms which it affected. Fungicide is a general term thatgiven to all chemicals which are used to take under control the fungus that infect on plants. Luna Experience SC-400 is a new generation fungicide that combinated with Fluopyram and Tebuconazole. Tebuconazole is a triazole fungicide. Tebuconazole was tested in vitro for its potential genotoxic and cytotoxic effects on cultured bovine peripheral lymphocytes. Fluopyram is a benzamide fungicide. Genotoxic effects of fluopyram was examined and reported in rats. In this study, We used three different doses (5 µg/kg, 10 µg/kg, 20 µg/kg) of Luna Experience-EC 400 during 30 day at 48 h intervals by gavage in rats. We investigated the genotoxicty and cytotoxicty of Luna Experience-EC 400 using comet assay and micronucleus test in rat bone marrow. We found that Luna Experience-EC 400 has genotoxic and cytotoxic potential in rat bone marrow. There is significant difference between control group and all the doses of Luna Experience-EC 400 (p<0.05) for comet assay levels and micronucleus frequency. In this study, we also investigated the PCE/total erythrocyte rate. All the doses of Luna Experience-EC 400 lead to decreasing the amount of PCEs. It is found that this decrease is statistically significant (p<0.05).