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Journals / Kocatepe Veteriner Dergisi / 2023 / Cilt: 16 Sayı: 3

Effect of Cryopreservation on DNA Damage and Various Sperm Parameters in the Post-Mortem Obtained Buffalo Sperm

Mandalardan Postmortem Elde Edilen Spermalarda Dondurmanın DNA Hasarı ve Bazı Spermatolojik Parametreler Üzerine Etkisi

Eser AKAL
Murat SELCUK
Pages
432–438
Publication Date
2023-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

More comprehensive studies should be performed to determine the impact of fertility due to the semen DNA damage and minimize the DNA damage in the freezing-thawing semen samples.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to investigate the effects of freezing on DNA damage and some sperm parameters in epididymal sperm obtained from the buffalo testicles after slaughtering.
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Key Findings & Data:Epididymal semen examination of 50 male Anatolian buffaloes (three years old or older) was performed after being obtained from slaughterhouses.
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Conclusion & Impact:More comprehensive studies should be performed to determine the impact of fertility due to the semen DNA damage and minimize the DNA damage in the freezing-thawing semen samples.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Anadolu mandası · DNA hasarı · Epididimal

Abstract

This study aimed to investigate the effects of freezing on DNA damage and some sperm parameters in epididymal sperm obtained from the buffalo testicles after slaughtering. Epididymal semen examination of 50 male Anatolian buffaloes (three years old or older) was performed after being obtained from slaughterhouses. Sperm progressive motility (%), viability (%), abnormal sperm (%), and sperm DNA damage (%) were analyzed. The study found significant differences (p<0.001) between fresh and thawed semen on the progressive motility, viability, and mid-piece, tail, and total abnormality rates. It was determined that the difference between the DNA damage values in fresh and thawed semen was significant (p<0.0001). Progressive motility rates of fresh and thawed semen were consistent with the studies conducted. It is thought that epididymal buffalo semen obtained post-mortem can be used in biotechnological methods. This research will contribute to further studies to increase the Anatolian buffalo population. More comprehensive studies should be performed to determine the impact of fertility due to the semen DNA damage and minimize the DNA damage in the freezing-thawing semen samples.

Özet

Bu çalışmada, kesim sonrası manda testislerinden elde edilen epididimal spermada dondurma işleminin DNA hasarı ve bazı spermatolojik parametreler üzerindeki etkisinin araştırılması amaçlanmıştır. 50 adet erkek Anadolu mandasının (3 ve üzeri) testisleri mezbahanelerden kesim sonrası temin edildikten sonra elde edilen epididimal sperma progresif motilite (%), canlılık (%), anormal spermatozoa oranı (%) ve spermatozoon DNA hasarı (%) yönünden analiz edildi. Taze ve çözdürülmüş sperma arasında progresif motilite, canlılık, orta kısım, kuyruk ve toplam anormallik oranlarında önemli farklılıklar buldu (p<0,001). Taze ve çözdürülmüş spermada arasındaki DNA hasarı sonuçlarının anlamlı derecede farklı olduğu belirlendi (p<0,0001). Taze ve çözdürülmüş spermadaki progresif motilite oranları yapılan çalışmalarla uyumluydu. Çalışma sonucunda elde edilen veriler ışığında postmortem olarak elde edilen epididimal manda spermasının biyoteknolojik yöntemlerde kullanılabileceği düşünülmektedir. Bu araştırma, Anadolu manda popülasyonunun artırılmasına yönelik daha sonraki çalışmalara katkı sağlayacaktır. Spermatozoonlarda dondurma işlemi sonucunda şekillenen DNA hasarının belirlenmesi ve en aza indirilmesi için daha kapsamlı çalışmalar yapılmalıdır.

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Cite This Article

AKAL, E., SELCUK, M. (2023). Effect of Cryopreservation on DNA Damage and Various Sperm Parameters in the Post-Mortem Obtained Buffalo Sperm. Kocatepe Veteriner Dergisi, Cilt: 16 Sayı: 3, 432–438. https://ajindex.com/articles/968149

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References (11)

  1. Ames, B. N., Motchnik, P. A., Fraga, C. G., Shigenaga, M. K., & Hagen, T. M. (1994). Antioxidants prevention of birth defects and cancer. In: Male-mediated development toxicology, Reproductive Biology. Springer, Boston. 243-259.
  2. Arriola, J. R., & Foote, H. (1987). Glycerolation and thawing effects on bull spermatozoa frozen in detergent-treated egg yolk and whole egg extenders. J Dairy Sci. 70: 1664-1670.
  3. Borghese, A., & Mazzi, M. (2005). Buffalo population and strategies in the world. In: Buffalo production and research, Reu Technical Series 67. 1-39.
  4. Chatterjee, S., De Lamirande, E., & Gagnon, C. (2001). Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol Reprod Dev. 60: 498–506.
  5. Cummins, J. M., Jequier, A. M., & Kan, R. (1994). Molecular biology of human male infertility: Linked with aging, mitochondrial genetics, and oxidative stress? Mol Reprod Dev. 37: 345-362.
  6. Donnelly, E. T., O'connell, M., Mcclure, N., & Lewis, S. E. (2000). Differences in nuclear DNA fragmentation and mitochondrial integrity of semen and prepared human spermatozoa. Hum Reprod. 15(7): 1552-1561.
  7. El-Kon, I., & Darwish, A. S. (2011). Effect of glutathione (GSH) on microscopic parameters, DNA integrity in Egyptian buffalo semen during liquid and frozen storage. J Reprod Infertil. 2(3): 32-40.
  8. Fraser, L., & Strzezek, J. (2004). The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C. Folia Histochem Cytobiol. 42: 49-55.
  9. Guerrero, C. A. (2006). Cryopreservation and intracytoplasmic sperm injection with bovine epididymal spermatozoa. PhD Thesis, Louisiana State University, Louisiana, USA. 216. Herold, F. C., Aurich, L. E., & Gerber, D. (2004a). Epididymal sperm from the African buffalo (Syncerus Caffer) can be frozen successfully with Andromed® and with TriladylTM but the addition of bovine seminal plasma is detrimental. Theriogenology. 61: 715–724.
  10. Herold, F. C., De Haas, K., Colenbrander, B., & Gerber, D. (2006). Comparison of equilibration times when freezing epididymal sperm from African buffalo (Syncerus Caffer) using TriladylTM or Andromed®. Theriogenology. 66: 1123–1130.
  11. Herold, F. C., De Haas, K., Cooper, D., Colenbrander, B., Nöthling, J. O., Theunisen, W., Spillings, B., & Gerber, D. (2004b). Comparison of three different media for freezing of epididymal sperm from the African buffalo (Syncerus Caffer) and influence of equilibration time on the post-thaw sperm q