Quantification of MMP-2 and TIMP-1 expressions in breast cancer

Objective & Hypothesis:Background and Aims: MMP-2 and TIMP-1 are vital molecules in the remodeling of the extracellular matrix, and they have a critical role in the metastatic process of breast cancer.

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Key Findings & Data:This study aimed to detect expression levels of MMP-2 and TIMP-1 genes by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and identify their potential roles in breast cancer prognosis.

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Conclusion & Impact:MMP-2 and TIMP-1 expression patterns will also aid in the identification of more precise and targeted subgroups of breast cancer.

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Breast cancer · MMP2 metalloproteinase · TIMP-1

Abstract

Background and Aims: MMP-2 and TIMP-1 are vital molecules in the remodeling of the extracellular matrix, and they have a critical role in the metastatic process of breast cancer. This study aimed to detect expression levels of MMP-2 and TIMP-1 genes by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and identify their potential roles in breast cancer prognosis. Methods: MMP-2 and TIMP-1 gene expression levels in 17 breast cancer tumor tissues and normal breast tissue were examined. The expression levels of MMP-2 and TIMP-1 were analyzed by qRT-PCR. The association between the expression levels of MMP-2 and TIMP-1 and clinicopathological manifestations of breast cancer was examined. Results: Lower gene expression levels of MMP-2 and TIMP-1 were detected in tumors compared to the controls. A statistical correlation was not observed between the expression level of MMP-2, TIMP-1, and clinicopathological parameters (tumor grade, lymph node involvement, hormone receptor status). Conclusion: Our findings have been suggesting that expression profiles of MMP-2 and TIMP-1 might be independent prognostic and predictive biomarkers for breast cancer. These biomarkers are candidate molecules for personalized therapy. MMP-2 and TIMP-1 expression patterns will also aid in the identification of more precise and targeted subgroups of breast cancer.

Keywords

Breast cancer MMP2 metalloproteinase TIMP-1

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Cite This Article

KESKİN, S. E., AKKOYUNLU, D. S., YILMAZ, M., ŞİMŞEK, T., GÜLER, S., ÇİNE, N., CANTÜRK, Z., SAVLI, H. (2023). Quantification of MMP-2 and TIMP-1 expressions in breast cancer. İstanbul Journal of Pharmacy, Cilt: 53 Sayı: 2, 211–218. https://ajindex.com/articles/963646

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