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Journals / Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2023 / Cilt: 25 Sayı: 2

Investigation of the effects of some drugs on sheep Paraoxonase-1

Bazı ilaçların koyun Paraoksonaz-1 üzerindeki etkilerinin araştırılması

Hatice Yüksel(BALIKESİR ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, KİMYA (DR))0000-0001-6436-9120
Adem Ergün(BALIKESİR ÜNİVERSİTESİ, BİLİM VE TEKNOLOJİ UYGULAMA VE ARAŞTIRMA MERKEZİ)0000-0003-4647-6058
Mikail Arslan(BANDIRMA ONYEDİ EYLÜL ÜNİVERSİTESİ, SUSURLUK MESLEK YÜKSEKOKULU)0000-0002-7882-9586
Oktay Arslan(BALIKESİR ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, KİMYA BÖLÜMÜ, BİYOKİMYA ANABİLİM DALI)0000-0002-2549-4997
Pages
483–488
Publication Date
2023-07-07
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to these values, furosemide (IC50 = 9.87 mM) was found to be a stronger inhibitor than enrofloxacin (IC50 = 42.21 mM).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Paraoxonase-1 (PON1, EC 3.1.8.1) is a metalloenzyme that has important roles in many metabolic processes.
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Key Findings & Data:PON1 (mPON1) was isolated from Karacabey Merino sheep in this study using the hydrophobic interaction method.
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Conclusion & Impact:According to these values, furosemide (IC50 = 9.87 mM) was found to be a stronger inhibitor than enrofloxacin (IC50 = 42.21 mM).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Paraoksonaz · inhibisyon · saflaştırma

Abstract

Paraoxonase-1 (PON1, EC 3.1.8.1) is a metalloenzyme that has important roles in many metabolic processes. PON1 (mPON1) was isolated from Karacabey Merino sheep in this study using the hydrophobic interaction method. The effects of enrofloxacin and furosemide on the pure enzyme were investigated. The drugs used in the study were found to inhibit the mPON1 enzyme at various levels. The IC50 values of the drugs were calculated from the related graphics, and their inhibition power was determined. According to these values, furosemide (IC50 = 9.87 mM) was found to be a stronger inhibitor than enrofloxacin (IC50 = 42.21 mM).

Özet

Paraoksonaz-1 (PON1, EC 3.1.8.1) birçok metabolik süreçlerde önemli rollere sahip bir metaloenzimdir. Bu çalışmada Karacabey Merinos koyunundan hidrofobik etkileşim tekniği ile PON1 (mPON1) saflaştırılmıştır. Saf enzim üzerinde enrofloksasin ve furosemid ilaçlarının etkileri araştırılmıştır. Çalışmada kullanılan ilaçların, mPON1 enzimini farklı düzeylerde inhibe ettiği bulunmuştur. İlaçların IC50 değerleri ilgili grafiklerden hesaplanarak inhibisyon güçleri saptanmıştır. Bu değerlere göre furosemid (IC50 = 9,87 mM)’in enrofloksasin (IC50 = 42,21 mM)’den daha güçlü inhibitör olduğu tespit edilmiştir.

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Cite This Article

Yüksel, H., Ergün, A., Arslan, M., Arslan, O., *, A. E. (2023). Investigation of the effects of some drugs on sheep Paraoxonase-1. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 25 Sayı: 2, 483–488. https://doi.org/10.25092/baunfbed.1205406

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