SavedAdd your journal

Journals / Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi / 2023 / Cilt: 9 Sayı: 1

Determination of Antibacterial and Antibiofilm Activities for Laurel (Laurus nobilis L.) Essential Oil Against the Fish Pathogen Pseudomonas Species

Defne (Laurus nobilis L.) Uçucu Yağının Balık Patojeni Pseudomonas Türlerine Karşı Antibakteriyel ve Antibiyofilm Aktivitelerinin Belirlenmesi

Serdar BEKTAŞ
Murat ÖZDAL
Sümeyra GÜRKÖK
Pages
25–33
Publication Date
2023-06-27
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Based on its demonstrated antibacterial and antibiofilm potential, LEO holds promise as a prospective source of antibacterial agents.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The essential oil derived from Laurus nobilis exhibits a high concentration of bioactive components, imparting various therapeutic characteristics.
📊
Key Findings & Data:Gas chromatography-mass spectrometry (GC-MS) analysis revealed that 1,8-cineole (%48.43) and α-terpinyl acetate (14.78) were the major compounds present in the essential oil (EO).
💡
Conclusion & Impact:Based on its demonstrated antibacterial and antibiofilm potential, LEO holds promise as a prospective source of antibacterial agents.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:antibakteriyel aktivite · antibiyofilm aktivite · uçucu yağ

Abstract

The essential oil derived from Laurus nobilis exhibits a high concentration of bioactive components, imparting various therapeutic characteristics. This study aimed to analyze the chemical composition of the essential oil extracted from Laurus nobilis leaves as well as its biological properties, including its antipseudomonal and antibiofilm actions. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that 1,8-cineole (%48.43) and α-terpinyl acetate (14.78) were the major compounds present in the essential oil (EO). While, the minimum inhibitory concentration (MIC) values of Laurus nobilis essential oils (LEO) against P. fluorescens and P. putida were determined as 31.25 µg/mL, it was 62.5 µg/mL for P. aeruginosa. LEO, at a MIC level of 31,25µg/mL, exhibited significant inhibition of Pseudomonas species biofilm formation except for P. aeruginosa. Based on its demonstrated antibacterial and antibiofilm potential, LEO holds promise as a prospective source of antibacterial agents.

Özet

Yüksek biyoaktif bileşen konsantrasyonuna sahip olması nedeniyle Defne (Laurus nobilis) esansiyel yağının, çeşitli terapötik özelliklere sahip olduğu bilinmektedir. Bu çalışma ile Laurus nobilis yapraklarından ekstrakte edilen esansiyel yağın kimyasal bileşiminin yanı sıra antipsödomonal ve antibiyofilm etkileri de dahil olmak üzere biyolojik özelliklerinin analiz edilmesi amaçlanmıştır. Minimum İnhibasyon Konsantrasyonu (MİK) değerlerinin, P. fluorescens için 31,25 µg/mL, P. putida için 31,25 µg/mL ve P. aeruginosa için 62,5 µg/mL olduğu tespit edilmiştir. P. fluorescens ve P. putida biyofilmlerinin, MİK altı dozlarda Laurus nobilis esansiyel yağının anti-biyofilm aktivitesi tarafından baskılandığı görülmüştür. Sonuçlar, minimum inhibasyon konsantrasyonlarında esansiyel yağın, Pseudomonas türü biyofilm oluşumunu etkili bir şekilde önlediğini ortaya koymuştur. Sonuç olarak, Laurus nobilis esansiyel yağlarının, antibiyofilm potansiyeline sahip muhtemel antibakteriyel ajan kaynakları olduğu sonucuna varılmıştır.

🔖

Cite This Article

BEKTAŞ, S., ÖZDAL, M., GÜRKÖK, S. (2023). Determination of Antibacterial and Antibiofilm Activities for Laurel (Laurus nobilis L.) Essential Oil Against the Fish Pathogen Pseudomonas Species. Menba Kastamonu Üniversitesi Su Ürünleri Fakültesi Dergisi, Cilt: 9 Sayı: 1, 25–33. https://ajindex.com/articles/946455

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Alfred, O., Shaahu, A., Orban, D. A., & Egwenomhe, M. (2020). An overview on understanding the basic concept of fish diseases in aquaculture. IRE Journals, 4(6), 83-91.
  2. Algammal, A. M., Mabrok, M., Sivaramasamy, E., Youssef, F. M., Atwa, M. H., El-Kholy, A. W., Hetta, H. F., & Hozzein, W. N. (2020). Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and blaTEM, blaCTX-M, and tetA antibiotic-resistance genes. Scientific Reports, 10(1), 15961. https://doi.org/10.1038/s41598-020-72264-4
  3. Arumugam, G., Swamy, M. K., & Sinniah, U. R. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules, 21(4), 369. https://doi.org/10.3390/molecules21040369
  4. Assefa, A., & Abunna, F. (2018). Maintenance of Fish Health in Aquaculture: Review of Epidemiological Approaches for Prevention and Control of Infectious Disease of Fish. Veterinary medicine international, 2018, 5432497. https://doi.org/10.1155/2018/5432497
  5. Austin, B., & Austin, D.A. (2012). Pseudomonadaceae Representatives. In: Bacterial Fish Pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4884-2_10
  6. Badawy, M. E., Marei, G. I. K., Rabea, E. I., & Taktak, N. E. (2019). Antimicrobial and antioxidant activities of hydrocarbon and oxygenated monoterpenes against some foodborne pathogens through in vitro and in silico studies. Pesticide Biochemistry and Physiology, 158, 185-200. https://doi.org/10.1016/j.pestbp.2019.05.008
  7. Bektaş, S., & Özdal, M. (2022). Antimicrobial Activity of Eucalyptus (Eucalyptus camaldulensis) Essential Oil Against Fish Pathogen Bacterium, Aeromonas caviae. Marine Science and Technology Bulletin, 11(4), 467-474. https://doi.org/10.33714/masteb.1184165
  8. Bektaş, S., & Ayik, O. (2009). Hematological parameters and erythrocyte osmotic fragility in rainbow trout, Oncorhynchus mykiss, experimentally infected with Pseudomonas putida. Journal of Fisheries and Aquatic Science, 4(5), 246-253. https://doi.org/10.3923/jfas.2009.246.253
  9. Caputo, L., Nazzaro, F., Souza, L. F., Aliberti, L., De Martino, L., Fratianni, F., & De Feo, V. (2017). Laurus nobilis: Composition of essential oil and its biological activities. Molecules, 22(6), 930. https://doi.org/10.3390/molecules22060930
  10. Chen, J., Rossman M.L., & Pawar, D.M. (2007). Attachment of enterohemorragic Escherichia coli to the surface of beef and a culture medium. LWT-Food Science and Technology, 40(2), 249-254. https://doi.org/10.1016/j.