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Journals / Sakarya University Journal of Science / 2020 / Cilt: 24 Sayı: 1

Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Ertan Ermiş
Ecem Poyraz
Enes Dertli
Mustafa Tahsin Yılmaz
Pages
151–160
Publication Date
2020-02-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Response surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25.
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Key Findings & Data:The effects of three variables, temperature (30, 36 and 42 °C), incubation time (18, 33 and 48 h) and initial pH of growth medium (4.5, 5.5 and 6.5) were studied.
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Conclusion & Impact:Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Lactic acid bacteria · exopolysaccharide · response surface methodology

Abstract

Response surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables, temperature (30, 36 and 42 °C), incubation time (18, 33 and 48 h) and initial pH of growth medium (4.5, 5.5 and 6.5) were studied. Under optimum growth conditions, the amount of exopolysaccharide derived from Lactobacillus brevis E25 ranged from 10 to 35 gL-1. The size of EPS molecules ranged from 105 to 107 Da. Infra red spectrum analysis showed characteristics absorption peaks indicating the presence of -OH, C=O and C-H groups. Furthermore, only glucose was detected as monosaccharide in exopolysaccharide structure, revealing that the structure of exopolysaccharide is a homopolymeric glucan type. Based on the differential scanning calorimeter thermograms, exopolysaccharide’s melting temperature was observed around 116 °C.

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Cite This Article

Ermiş, E., Poyraz, E., Dertli, E., Yılmaz, M. T. (2020). Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization. Sakarya University Journal of Science, Cilt: 24 Sayı: 1, 151–160. https://ajindex.com/articles/941948

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