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Journals / European Journal of Research in Dentistry / 2023 / Cilt: 7 Sayı: 1

In vitro Evaluation of Cell Viability and Cytotoxicity Effects of Chlorhexidine on Human Gingival Fibroblasts

Klorheksidinin İnsan Dişeti Fibroblastları Üzerindeki Hücre Canlılığı ve Sitotoksisite Etkinliğinin İn vitro Koşullarda Değerlendirilmesi

Gözdem BAYRAKTAR
Ayse YİLMAZ
Hafize ÖZTÜRK ÖZENER
Pages
27–32
Publication Date
2023-04-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The aim of this study was to evaluate the cell viability and cytotoxicity effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts (HGF) in vitro.
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Key Findings & Data:Materials and Methods: A 0.2 % CHX solution with a neutral pH was tested at 30 second and 2 minute intervals.
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Conclusion & Impact:Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:klorheksidin · fibroblast · in vitro

Abstract

Objectives: The aim of this study was to evaluate the cell viability and cytotoxicity effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts (HGF) in vitro. Materials and Methods: A 0.2 % CHX solution with a neutral pH was tested at 30 second and 2 minute intervals. Cell viability and cytotoxicity against HGF were determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the lactate dehydrogenase (LDH) test. Results were evaluated by calculating means and standard deviations using the two-way ANOVA test (p<0.05). Results: According to the MTT test results, it was observed that a 0.2 % CHX solution decreased cell viability over time. According to the LDH test results, the cytotoxic efficacy of 0.2 % CHX was higher compared to the control group, but did not show a statistically significant difference over time. Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.

Özet

Amaç: Bu in vitro çalışmada, % 0,2’lik klorheksidin (CHX) solüsyonunun, insan dişeti fibroblastları (HGF) üzerindeki hücre canlılığı ve sitotoksisite etkinliklerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: 30 saniye ve 2 dakikalık zaman aralıklarında, nötr pH değerindeki % 0,2’lik CHX solüsyonu kullanılarak çalışılmıştır. Hücre canlılığı ve sitotoksisitesi, HGF’ye karşı 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) testi ve laktat dehidrogenaz (LDH) testi kullanılarak değerlendirilmiştir. Sonuçlar ortalama ve standart sapma değerleri kullanılarak two way ANOVA testiyle istatistiksel olarak değerlendirilmiştir (p<0,05). Bulgular: MTT testi sonuçlarına göre, % 0,2 CHX solüsyonunun zamanla hücre canlılığını azalttığı gözlendi. LDH testi sonuçlarına göre ise % 0,2 CHX’in sitotoksik etkinliğinin kontrol grubuna kıyasla arttığı ancak zaman içinde istatistiksel olarak anlamlı bir fark göstermediği gözlendi. Sonuç: Bu in vitro çalışmanın sınırları dahilinde, % 0,2 CHX solüsyonu, 30 sn ve 2 dk’lık kısa maruz kalma sürelerinde, HGF hücre canlılığını azalttı ve HGF üzerine sitotoksik etkinlik gösterdi.

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Cite This Article

BAYRAKTAR, G., YİLMAZ, A., ÖZENER, H. Ö. (2023). In vitro Evaluation of Cell Viability and Cytotoxicity Effects of Chlorhexidine on Human Gingival Fibroblasts. European Journal of Research in Dentistry, Cilt: 7 Sayı: 1, 27–32. https://ajindex.com/articles/918929

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