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Journals / Journal of Anatolian Environmental and Animal Sciences / 2020 / Cilt: 5 Sayı: 2

Effect of different genetic manipulations on the production of xylanase enzyme in Streptomyces strains

Streptomyces suşlarında farklı genetik manipulasyonların ksilinaz enzim üretimi üzerine etkisi

Fatma Esen SARIGÜLLÜ ÖNALAN
Pages
224–230
Publication Date
2020-06-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study also indicated that the enzyme activity can be improved by protoplast fusion technique

📌 3 Key Takeaways

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Objective & Hypothesis:Soil samples were collected from five different areas of University of Çukurova and 3 Streptomyces strains were isolated.
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Key Findings & Data:The xylanase activity (at the end of the seventh day) of S40, S47 and S48 strains were found as 50,92U/mL, 49,51U/mL and 47,79U/mL respectively.
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Conclusion & Impact:This study also indicated that the enzyme activity can be improved by protoplast fusion technique
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Streptomyces · ksilanaz · protoplast füzyonu

Abstract

Soil samples were collected from five different areas of University of Çukurova and 3 Streptomyces strains were isolated. The xylanase activity (at the end of the seventh day) of S40, S47 and S48 strains were found as 50,92U/mL, 49,51U/mL and 47,79U/mL respectively. After doing the protoplast fusion among these strains which have the highest xylanase activity, 9 strains which have different colony morphologies, were selected. The xylanase activity of (40-48) R2 and (47-48) R3 strains were increased in comparison with their parents, by 25,9% and 31,9% respectively. This study also indicated that the enzyme activity can be improved by protoplast fusion technique

Özet

Çukurova Üniversitesi’nin 5 farklı bölgesinden alınan toprak örneklerinden 3 Streptomyces suşu izole edildi. S40, S47, S48 suşlarının 7 gün sonundaki ksilanaz aktivitesi sırasıyla 50,92 U/mL, 49,51 U/mL, 47,79 U/mL tespit edilmiştir. Yüksek ksilanaz aktivitesi gösteren bu suşlar arasında gerçekleştirilen protoplast füzyon çalışması sonucu farklı koloni morfolojsi gösteren 9 suş seçilmiştir. (40-48)R2 ve (47-48)R3 suşlarının ksilanaz aktivitesi ebeveyn suşlara göre sırasıyla %25,9 ve % 31,9 oranında arttığı tespit edilmiştir. Protoplast füzyon tekniğiyle enzim aktivitesinin geliştirilebileceği gösterilmiştir.

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Cite This Article

ÖNALAN, F. E. S. (2020). Effect of different genetic manipulations on the production of xylanase enzyme in Streptomyces strains. Journal of Anatolian Environmental and Animal Sciences, Cilt: 5 Sayı: 2, 224–230. https://ajindex.com/articles/907722

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References (1)

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