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Journals / Academic Platform Journal of Engineering and Smart Systems / 2017 / Cilt: 5 Sayı: 3

DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile

2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması

Ali ARSLANTAS
Mehmet Salih AĞIRTAŞ
Pages
1–9
Publication Date
2017-10-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The cyclic voltammetric results show one half- reversible reduction oxidation wave.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, binding activities of the previously synthesized cobalt (II) compound (PcCo) with calf thymus-DNA containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile were investigated to determine binding activities by using UV-Vis absorption, fluorescence titration methods.
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Key Findings & Data:Absorption titration spectra of PcCo in DMF give peaks at about 670 nm for Q-band absorption and about 340 nm for B-band absorption in the absence of calf thymus-DNA.
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Conclusion & Impact:The cyclic voltammetric results show one half- reversible reduction oxidation wave.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:DNA · DNA-bağlanma · Ftalosiyaninler

Abstract

In this study, binding activities of the previously synthesized cobalt (II) compound (PcCo) with calf thymus-DNA containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile were investigated to determine binding activities by using UV-Vis absorption, fluorescence titration methods. Absorption titration spectra of PcCo in DMF give peaks at about 670 nm for Q-band absorption and about 340 nm for B-band absorption in the absence of calf thymus-DNA. Emission intensities of PcCo in presence of calf thymus-DNA, PcCo gives emission at about 545 nm. Thermal denaturation profile study was systematically studied by UV/Vis spectroscopic technique and melting point temperaturewas found around 7.87 oC for Co (II) phthalocyanine compound. The reduction oxidation behavior of PcCo with calf thymus-DNA was investigated using cyclic voltammetry (CV). Cathodic peak potential and anodic peak potential for PcCo were recorded to be 0.84, -0.36 V for EPc and 0.88 V, 0.15 V, and -0.69 V EPa. The cyclic voltammetric results show one half- reversible reduction oxidation wave. The shift in Epc and Epa potentials indicates that PcCo shows strong interaction with calf thymus-DNA. Further to understand binding activities of PcCo with calf thymus-DNA was studied by using electrophoresis technique. Electrophoresis findings showed that there is decrease in the intensity of CT-DNA bands. All the findings show that PcCo interacts strongly with DNA molecule via an intercaltion binding.

Özet

2,10, 16, 24-tetrakis 4- (2-fenoksi-1,3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren önceden sentezlenmiş kobalt (II) bileşiğinin (PcCo) CT-DNA ile bağlanma özellikleri UV/Vis absorpsiyon, floresan titrasyonu yöntemleri kullanılarak bağlanma özelliklerini belirlemek için araştırıldı. Termal denatürasyon profili çalışması UV-Vis spektroskopik tekniği ile sistematik olarak incelenmiş ve Co (II) ftalosiyanin bileşiği için erime noktası sıcaklık değeri 7.87 oC civarında bulunmuştur. CT-DNA yokluğunda ve varlığında PcCo'in redüksiyon oksidasyon davranışı, dönüşümlü voltametri (CV) ile incelendi. Dönüşümlü (cyclic) voltametri sonuçlar, bir yarı-tersinir redaksiyon oksidasyon dalgasını göstermektedir. Epc ve Epa potansiyelindeki değişim, PcCo'in CT-DNA'yla güçlü etkileşim gösterdiğini önermektedir. PcCo'in CT-DNA ile olan etkileşimi ayrıca jel elektroforez yöntemi kullanılarak araştırılmıştır. Jel elektroforezi sonuçları, CT-DNA bantlarının yoğunluğunda azalma olduğunu gösterdi. Tüm bulgular, PcCo ile CT-DNA molekülü arasındaki etkileşimin interkalatif bağlanma türü olduğunu göstermektedir.

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Cite This Article

ARSLANTAS, A., AĞIRTAŞ, M. S. (2017). DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile. Academic Platform Journal of Engineering and Smart Systems, Cilt: 5 Sayı: 3, 1–9. https://ajindex.com/articles/899631

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