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Journals / Kastamonu Üniversitesi Orman Fakültesi Dergisi / 2023 / Cilt: 23 - Sayı: 1

Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye

Bitki Büyüme Düzenleyicilerinin Crepis bithynica (Asteraceae)’nın Sürgün Çoğaltımı Üzerine Etkileri: Türkiye’de Tehlike Altında Nadir Bir Tür

Tuğba ERGİN
Hüseyin İNCEER
Pages
47–51
Publication Date
2023-03-24
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim of study: In this study, the effects of plant growth regulators (PGRs) on shoot multiplication of Crepis bithynica Boiss. (Asteraceae), an endangered rare species in Türkiye were investigated.
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Key Findings & Data:Morphological changes of propagated plants were monitored during 21 days in vitro cultures.
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Conclusion & Impact:Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Crepis bithynica · Bitki Büyüme Düzenleyicileri · Sürgün Çoğaltımı

Abstract

Aim of study: In this study, the effects of plant growth regulators (PGRs) on shoot multiplication of Crepis bithynica Boiss. (Asteraceae), an endangered rare species in Türkiye were investigated. Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park. Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog (MS) basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures. Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively. Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.

Özet

Çalışmanın amacı: Bu çalışmada, bitki büyüme düzenleyicilerinin (PGRs) Türkiye’de tehlike altında nadir bir tür olan Crepis bithynica Boiss. (Asteraceae)’nın sürgün çoğaltımı üzerine etkileri araştırılmıştır. Çalışma alanı: Bitki örnekleri, Kastamonu Ilgaz Dağı Milli Parkı’ndan toplanmıştır. Materyal ve yöntem: Nodal segmentler eksplant olarak kullanıldı ve bunlar farklı bitki büyüme düzenleyicileri ile desteklenmiş Murashige and Skoog (MS) temel besiyerlerinde kültüre edildi. Çoğaltılan bitkilerdeki morfolojik değişmeler 21 gün boyunca in vitro kültürlerde izlendi. Temel sonuçlar: Tüm bitki büyüme düzenleyicileri kallus oluşturmaksızın sürgün büyüme ve gelişmesini teşvik etmişlerdir. Bununla birlikte, eksplant başına en yüksek sürgün sayısı ve sürgün uzunluğu, sırasıyla 4.4 µM 6-BA ile 0.5 µM NAA ve 4.9 µM 2iP ile 0.5 µM IBA içeren MS besiyerlerinde elde edilmiştir. Araştırma vurguları: Bu çalışma, C. bithynica’nın sürgün çoğaltımında PGR’lerin etkileri üzerine ilk çalışmadır ve elde edilen sonuçlar türün mikroçoğaltımı için kullanılabilir.

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Cite This Article

ERGİN, T., İNCEER, H. (2023). Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye. Kastamonu Üniversitesi Orman Fakültesi Dergisi, Cilt: 23 - Sayı: 1, 47–51. https://ajindex.com/articles/886896

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