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Journals / İstanbul Tıp Fakültesi Dergisi / 2023 / Cilt: 86 - Sayı: 1

EXPLORING MITOMIRS IN BREAST CANCER: AN IN-VITRO STUDY OF THEIR EMERGING ROLES

MEME KANSERİNDE MİTOMİR’LERİN TANIMLANMASI: İN VİTRO ÇALIŞMA İLE ROLLERİNİN GÖSTERİLMESİ

Pervin Elvan TOKGÜN
Ayşe Gaye TOMATIR
Pages
69–77
Publication Date
2023-01-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Identifying new mitomiRs can provide significant contributions and illuminate the molecular mechanism underlying mitomiR biogenesis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Breast cancer is associated with a 5% genetic predisposition in women.
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Key Findings & Data:Mitochondria play a role in important cellular events such as metabolism, cell death, and inflammation.
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Conclusion & Impact:Conclusion: Identifying new mitomiRs can provide significant contributions and illuminate the molecular mechanism underlying mitomiR biogenesis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:meme kanseri · mitomiR · MACS

Abstract

Objective: Breast cancer is associated with a 5% genetic predisposition in women. Mitochondria play a role in important cellular events such as metabolism, cell death, and inflammation. Recent studies have highlighted precursor micro-RNA (pre-miRNA) to be located in mitochondria as well as mature miRNA. This study aims to reveal the occurrence of mitochondrial miRNA (mitomiR) in breast cancer cells. Materials and Methods: The study has prepared the mitochondrial fractions using the magnetic-activated cell sorting (MACS) method and performed small RNA (sRNA) sequencing. Results: The study has identified known and novel mitomiR sequences aligned to the mitochondrial genome. Conclusion: Identifying new mitomiRs can provide significant contributions and illuminate the molecular mechanism underlying mitomiR biogenesis.

Özet

Amaç: Kadınlarda meme kanseri %5 oranında genetik yatkınlıkla ilişkilidir. Mitokondri, metabolizma, hücre ölümü ve inflamasyon gibi önemli hücresel olaylarda rol oynar. Son çalışmalar, olgun miRNA’ların yanı sıra pre-miRNA’ların mitokondride bulunduğunu göstermişlerdir. Bu çalışmada meme kanseri hücrelerinde mitomiR’lerin varlığını ortaya koymayı amaçladık. Gereç ve Yöntem: MACS yöntemi kullanılarak mitokondriyal fraksiyonlar hazırlandı ve küçük RNA dizilimi yapıldı. Bulgular: Mitokondriyal genoma hizalanmış bilinen ve yeni mitomiR dizilerini belirledik. Sonuç: Yeni mitomiR’lerin tanımlanması literatüre önemli katkılar sağlayabilir ve mitomiR biyogenezi moleküler mekanizmalar ile aydınlatılabilir.

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Cite This Article

TOKGÜN, P. E., TOMATIR, A. G. (2023). EXPLORING MITOMIRS IN BREAST CANCER: AN IN-VITRO STUDY OF THEIR EMERGING ROLES. İstanbul Tıp Fakültesi Dergisi, Cilt: 86 - Sayı: 1, 69–77. https://ajindex.com/articles/886661

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