AI Summary & Key Takeaways
AI InsightReal-time PCR with specific primers for PTGS2, CALR and KRT19 genes was used to determine the expression of these genes at the transcriptional level.Results: In the MDA-MB-231 cell line, a 14.92-fold increase in PTGS2 gene expression, a 1.45-fold increase in CALR gene expression and a 6.72-fold increase in expression of the KRT19 gene were found.Conclusions: It is predicted that the KRT19, CALR, PTGS2 genes, which are known to play a role in biological processes such as apoptosis resistance, metastasis, and angiogenesis in different solid cancers, may also play a role in the development of breast cancer and will have prognostic importance with further studies.Key words: Breast cancer, PTGS2 gene, CALR gene, KRT19 gene
📌 3 Key Takeaways
Abstract
Background: In this study, we aimed to determine the gene expressions of prostaglandin endoperoxide H synthase 2 (PTGS2), calreticulin (CALR), and keratin-19 (KRT19) genes at transcriptional level in MDA-MB-231 breast cancer cell line.Materials and Methods: RNA was isolated from MDA-MB-231 breast cancer cells grown in culture medium, followed by cDNA synthesis. Real-time PCR with specific primers for PTGS2, CALR and KRT19 genes was used to determine the expression of these genes at the transcriptional level.Results: In the MDA-MB-231 cell line, a 14.92-fold increase in PTGS2 gene expression, a 1.45-fold increase in CALR gene expression and a 6.72-fold increase in expression of the KRT19 gene were found.Conclusions: It is predicted that the KRT19, CALR, PTGS2 genes, which are known to play a role in biological processes such as apoptosis resistance, metastasis, and angiogenesis in different solid cancers, may also play a role in the development of breast cancer and will have prognostic importance with further studies.Key words: Breast cancer, PTGS2 gene, CALR gene, KRT19 gene
Özet
Amaç: Bu çalışmada MDA-MB-231 meme kanser hücre hattında prostaglandin endoperoksid H sentaz 2 (PTGS2), kalretikulin (CALR) ve keratin-19 (KRT19) genlerinin transkripsiyon düzeyindeki gen anlatımlarının belirlenmesi amaçlanmıştır.Materyal ve metod: Kültür ortamında çoğaltılan MDA-MB-231 meme kanser hücrelerinden RNA izolasyonu yapılmış ardından cDNA sentezi gerçekleştirilmiştir. PTGS2, CALR ve KRT19 genlerine spesifik primerler ile eş zamanlı PCR yapılarak, bu genlerin ifadesi transkripsiyonel seviyede belirlenmiştir.Bulgular: MDA-MB-231 hücre hattında PTGS2 gen ifadesinde 14,92 kat; CALR gen ifadesinde 1,45 kat; KRT19 geninin ifadesinde ise 6,72 kat artış olduğu saptanmıştır.Sonuç: Farklı solid kanserlerde, apoptoz direnci, metastaz, anjiyogenez gibi biyolojik süreçlerde rol aldığı bilinen KRT19, CALR, PTGS2 genlerinin meme kanseri gelişiminde de rol alabileceği ve ileride yapılacak detaylı çalışmalarla prognostik öneme sahip olacağı ön görülmektedir.Anahtar Kelimeler: Meme kanseri, PTGS2 geni, CALR geni, KRT19 geni